David L. Haviland, Ph.D.
Address and phone number withheld for anti-solicitation and junk mail/spam reasons – available upon request and confirmation that those seeking the information are genuine• starhopper457@gmail.com or david_haviland@hotmail.com
Objective: Seeking an opportunity to be a team player and join an interactive group of like minded professionals.
Employment
Assistant Professor
(1996-Present) University of Texas , Houston, H.S.C., Institute of Molecular Medicine Houston , Texas. Assistant Professor of Immunology. Basic science research in inate immunity involving GPCRs, expression, genetics. Current Director of the UT-Houston, HSC, Institute of Molecular Medicine Flow Cytometry and Fluorescent Microscope Laboratory. Safety officer. Becton-Dickinson FACS Vantage factory trained.
Research Assistant Professor
(1994-96), Washington University School of Medicine, Department of Pediatrics St. Louis , Missouri
Director of Pediatrics Research Flow Cytometer. Research Interest: Expression and Regulation of fMLF-R and C5a-R Chemotactic Receptors. Director, Clinical Immunology Laboratory, St. Louis Children's Hospital (1994-1995).
Instructor
(1992-94) - Washington University School of Medicine, Department of Pediatrics St. Louis , Missouri, Became the Director of Pediatrics Research Flow Cytometer. Research Interest: Expression and Regulation of fMLF-R and C5a-R Chemotactic Receptors.
Post Doctoral Research Fellow
(1988-92) - Washington University School of Medicine, Department of Pediatrics St. Louis , Missouri, Molecular Biology of human and murine C5 gene structures and deficiencies. Acquired skills in molecular biology.
(1987-88) - University of California at Riverside , Division of Biomedical Sciences Riverside , California, Taught undergraduate Microbiology course, Flow cytometry, and continued studies on CD44.
Education
Ph.D,. Biology - Years Attended (1983-87) - University of California at Riverside Riverside , California
Predoctoral Student and Teaching Assistant, Department of Biology. Universityof California , Riverside . Major Professor: Dr. Carl F. Ware. Dissertation Title: "Functional and Biochemical Properties of an 80,000 Mr Lymphoid Cell Surface Protein (p80)." Best Teaching Assistant of the Year (1983), Outstanding Research Award in Cellular Immunology and Flow Cytometry (1988).
M.A., Biology -Years Attended (1980-83) - University of California at Riverside Riverside , California
Graduate Student and Teaching Assistant, Department of Biology. University of California , Riverside . Major Professor: Dr. Bruce H. Devens. Master's Thesis Entitled: "A Study of the Binding Characteristics of Tritiated Phorbol Dibutyrate to Murine Splenocytes and Splenocyte Subpopulations".
B.A., Biology -Years Attended (1976-80) - University of California at San Diego La Jolla , California
Skills
Broad range of cellular, immunological, and molecular biology techniques including (but not limited to): tissue culture, lymphocyte and neutrophil isolation, stable and transient transfection, generation of hybridomas, cytotoxic T cell chromium release assays, thymidine incorporation assays, ELISA's, affinity purification of both polyclonal and monoclonal antibodies, cDNA and genomic library construction, genomic and cDNA cloning including gene ablation targeting vector construction, cDNA and genomic (cosmid and phage) library screening, PCR, RT-PCR.,automated DNA sequencing, contig assembly (Lasergene and Vector NTI), RNA techniques including isolation, primer extension and RNAse protection assays, mRNA isolation, calcium transient assays, phospholipase C assays (PLC), immunoprecipitation, SDS-PAGE, IEF, multiparameter flowcytometry, cell sorting (BD Vantage), immunofluorescence, tissue processing, immunohistochemistry, MS Word, Powerpoint, Wordperfect, Quatro, Presentations, Advanced Windows 98 and XP support, HTML, and computer hardware maintenance.
Editorial Services, Funding, & Publications
Ad Hoc Journal Reviews for: Journal of Immunology, Arthritis & Rheumatism, Protein Science, Circulation, European Journal of Biochemistry, and American Journal of Pathology.
GRANT SUPPORT
09/09/96-08/31/01- N.I.H.: United States Public Health Service Grant: "Human N-Formyl Peptide Receptor: Regulation and Genetics." 1R29GM56050-01, Principal Investigator-50% Effort. Avg. Annual Direct Cost - $70,000. Objectives: Characterize the expression, regulation, and genetics of the fMLF-R gene as well as determine role in mediating acute phase response.
07/01/99-06/30/03 - National Institute of Health: United States Public Health Service Grant: A Trauma Research Center , Program Project Grant, 2-P50-GM38529-11. Co-Investigator on Project I (Dr. Frank Moody, PI). Anticipated 10% Involvement/Effort to examine the role of complement, chemoattractants, and chemoattractant receptors in post-injury intestinal stasis (ileus) and resultant complications leading to multi-organ-system-failure (MOF).
09/30/01-07/31/06 - National Institute of Health: (NAIAD) United States Public Health Service Grant 1 RO1 HL68520-01 “Reactivation tuberculosis in A/J mice.” Co-Principal Investigator , 15%. The major goal of this project is to characterize latent tuberculosis in Complement C5 deficient A/J mice. Avg. Annual Direct Cost $250,000 /year
25 Peer reviewed published manuscripts, 1 in preparation. 21 Published Abstracts. (Selected manuscripts)
- Wetsel, R.A., D.T. Fleischer, and D.L. Haviland. 1990. Deficiency of the Murine Fifth Complement Component (C5): A Two Base Pair Gene Deletion in a 5' Exon. J. Biol. Chem., 265:2435-2440.
- Haviland, D.L., J.C. Haviland, D.T. Fleischer, and R.A. Wetsel. 1991. Structure of the Murine Fifth Complement Component (C5) Gene. A Large Highly Interrupted Gene with a Variant Donor Splice Site and Organizational Homology with the Third and Fourth Complement Component Genes. J. Biol. Chem., 266:11818-11825.
- Haviland, D.L., R.L. McCoy, W.T. Whitehead, H. Akama, E.P. Molmenti, A. Brown, J.C. Haviland, W.C. Parks, D.H. Perlmutter, and R.A. Wetsel. 1995. Cellular Expression of the C5a Anaphylatoxin Receptor (C5a-R): Demonstration of C5a-R on Liver and Lung Cells. J. Immunol., 154:1861-1869.
- McCoy, R., D.L. Haviland, E.P. Molmenti, T. Ziambaras, R.A. Wetsel, and D.H. Perlmutter. 1995. The N-Formylpeptide and Complement C5a Receptors are Expressed in Liver Cells and Mediate Hepatic Acute Phase Gene Regulation. J. Exp. Med., 182:207-217.
- Hollmann,T.J., D.L. Haviland, J. Kildsgaard, K. Watts, and R.A. Wetsel. 1998. Cloning, Expression, Sequence Determination, and Chromosome Localization of the Mouse Complement C3a Anaphylatoxin Receptor Gene. Mol. Immunol. 35:137-148.
- Baker, E.A., M.W. Vaughn, and D.L. Haviland. 2000. Choices in Transfection Methodologies: Transfection Efficiency Should Not Be the Sole Criterion. Focus, 22:31-33.
- Vaughn, M., R.J. Proske, and D.L. Haviland 2002. Identification, Cloning, and Functional Characterization of a Murine Lipoxin A4 Receptor Homologue Gene. J. Immunol. 169:3363-3369.
- Lee, L.Y.L. 1, Höök, M., Haviland, D.L., Wetsel, R. A., Yonter, E.O., Syribeys, P., Vernachio, J., and Brown, E.L. 2004. A secreted Staphylococcus aureus protein inhibits complement activation. J. Infect. Dis. 190:571-579.
- Zhong, S., Liu, C., Haviland, D.L., and B.B. Teng. 2005. Co-Expressing Apolipoprotien B mRNA Editing Enzyme and Scavenger Receptor, Class B, Type I Genes Mediated by a Helper-Dependent Adenoviral Vector. Atherosclerosis, 184(2):264-75.
- Mueller-Ortiz, S.L., Hollmann, T.J., Haviland, D.L., and Wetsel, R.A., 2006. Ablation of the complement C3a anaphylatoxin receptor causes enhanced killing of Pseudomonas aeruginosa in a mouse model of pneumonia. A. J. Physiol. Lung Cell Mol. Physiol. 291: L157-L16.
- Wang, D., Haviland, D.L., Burns, A.R., Zsigmond, E.,and Wetsel, R.A. 2007. A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells. Proc. Natl. Acad.of Sci., U.S.A. 104:4449-4454.
- Moulton, R.A., Mashruwala, M.A., Smith, A.K., Lindsey, D.R., Wetsel, R.A., Haviland, D.L., Hunter, R.L. and Jagannath, C. 2007. Complement C5a anaphylatoxin in an innate determinant of dendritic cell induced Th1 immunity to Mycobacterium bovis BCG infection in mice. J. Leukocyte Biol. 82(4):956-67.
COMPLETE CURRICULUM VITAE AND REFERENCES AVAILABLE UPON REQUEST